Qantas cancelled flight QF6 from Rome to Perth on Saturday after an outbreak of bed bugs on the aircraft.
The problem came to light on October 9, when passengers aboard the outbound service QF5 from Perth to Rome complained of being bitten by bugs. On medical advice they were given antihistamines, though the plane reportedly ran out of the medication.
The return flight was cancelled and the Dreamliner was booked in for cleaning. Qantas said passengers were given accommodation and rebooked on alternative flights within 48 hours of their original departure.
A spokesperson for the airline said: "We sincerely apologise to customers for the cancellation of Saturday's Rome to Perth service, which allowed our engineers and maintenance teams to carry out a deep clean of the aircraft after an issue was identified on a small number of economy seats."
The Perth-Rome route is a popular non-stop service that attracts passengers from Australia's east coast.