The new charges relate to the alleged wrongful detention of more than a dozen US citizens during Maduro's rule, according to CNN, which cited people familiar with the investigation. Prosecutors were also reported to be considering charging several other people over their alleged role in the torture of Americans.
CNN reported that charges could be announced as soon as Thursday, local time. A spokesperson for the Manhattan US Attorney's office, which brought the original drug trafficking case, declined to comment. Lawyers for Maduro and Flores did not immediately respond to requests for comment.
Maduro, 63, and Flores, 69, were captured in a US military operation at their home in Caracas in January and brought to New York to face drug trafficking charges. They have pleaded not guilty, and their trial is expected to begin in June. The couple has been detained in a federal jail in Brooklyn.
Washington previously accused Maduro's government of detaining Americans as leverage to extract concessions, such as relief from financial sanctions. US citizens classified as wrongfully detained during his tenure included five oil executives arrested on graft charges, who were freed in 2022 in exchange for two of Flores's nephews convicted in the US of drug trafficking.
Since Maduro's capture, Venezuela has been led by his former vice-president, Delcy Rodríguez, who has increased cooperation with the Trump administration.
Flores is due to appear in Manhattan federal court on Thursday, local time, where her lawyers are expected to request she be released from jail before the trial on health grounds. Prosecutors oppose her release.