The man was removed from the 4 Pines pub, on the corner of Noosa Drive and Sunshine Beach Road, about 2.30am on Saturday, police said. He was stopped by security when he tried to re-enter the venue.
He is then accused of punching a 27-year-old male security guard in the face before pushing the 60-year-old female guard down a set of stairs and fleeing the premises.
The 27-year-old guard from Pacific Paradise suffered a minor injury to his face, while the 60-year-old guard from Kin Kin was uninjured.
Police have released an image of a man they believe can help with their investigation into the alleged assault on October 10 and have asked the man, or anyone who recognises him, to contact them.